In vitro measurement of pollen tube growth inhibition.
نویسنده
چکیده
A method for estimating inhibition of pollen tube growth was developed. Pollen is placed in straight lines on an agar surface where it responds uniformly and predictably to aqueous solutions of germination-inhibiting substances located in wells at the ends of the lines. A scale of ratings, roughly corresponding to serial, doubled concentrations of inhibiting substances, was devised. Water-soluble organic solvents are relatively noninhibitory, salts are variable, and metabolic inhibitors have strong inhibitory effects. Pollens differ in their susceptibility to inhibition and in their response to particular substances.
منابع مشابه
Effects of Copper and Leadon Pollen Germination Traits in Almond Cultivars
There is minimal information about the effect of heavy metals such as lead and copper on pollen grains and pollen tubes of fruit trees. Fruit set of these plants are affected by different environmental, biological, physical and chemical factors. If one of these factors be abnormal, pollination, fertilization and fruit set and orchard yield will decrease. In polluted cities, this phenomenon is a...
متن کاملInhibition of in Vitro Pollen Tube Growth by Isolated S-Glycoproteins of Nicotiana alata.
Pollen from three S-genotypes of Nicotiana alata was grown in vitro in the presence of S-glycoproteins isolated from styles of the same three genotypes. Pollen germination was not affected by the presence of the S-glycoproteins, but pollen tube growth of all genotypes was inhibited. S2 pollen was preferentially inhibited by the S2-glycoprotein and S3 pollen by the S3-glycoprotein. The S6-glycop...
متن کاملSelf-incompatibility in the Iranian Almond Cultivar ‘Mamaei’ Using Pollen Tube Growth, Fruit Set and PCR Technique
Self-incompatibility has been studied by using controlled pollination, pollen tube growth and PCR methods in the Iranian almond ‘Mamaei.’. Pollen tube growth and fruit set following self and cross-pollination treatments were evaluated. The percentage of initial and final fruit set was determined for each treatment at 30 and 60 days after controlled pollination. Pollen germination and pollen ...
متن کاملIn vitro Arabidopsis pollen germination and characterization of the inward potassium currents in Arabidopsis pollen grain protoplasts.
The focus of this study is to investigate the regulatory role of K(+) influx in Arabidopsis pollen germination and pollen tube growth. Using agar-containing media, in vitro methods for Arabidopsis pollen germination have been successfully established for the first time. The pollen germination percentage was nearly 75% and the average pollen tube length reached 135 microm after a 6 h incubation....
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Plant physiology
دوره 49 6 شماره
صفحات -
تاریخ انتشار 1972